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{{/_source.additionalInfo}}Anti-ARF1 | ADP-ribosylation factor 1
(Cat#: AS08 325)
Description
- Immunogen: Recombinant GST fusion of full length of Arabidopsis thaliana ARF1 (P36397, AT2G47170)
- Host: Rabbit
- Clonality: Polyclonal
- Purity: Serum
- Format: Lyophilized
- Quantity: 50 µl
- Reconstitution: For reconstitution add 50 µl of sterile water
- Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
- Tested applications: Immunofluorescence (IF), Immunogold (IG), Western blot (WB)
- Recommended dilutions: 1 : 1000 (IF), 1 : 100 (IG), 1 : 1000 (WB)
- Expected | apparent MW: 21 kDa (Arabidopsis thaliana)
- Confirmed reactivity: Actinidia chinensis, Arabidopsis thaliana, Chlamydomonas reinhardtii, Elaeis sp. , Lilium longiflorum, Medicago truncatula, Nicotiana tabacum, Oryza sativa, Petunia hybrida cv Mitchell, Physcomitrium patens, Solanum tuberosum
- Not reactive in: Centella asiatica, Microsporidia sp.
- The ARF1 protein is localized to the Golgi apparatus and has a central role in intra-Golgi transport. It is a small GTPase that undergoes a GDP/GTP nucleotide exchange cycle and it is an important regulator of cellular trafficking.
- Mohr et al. (2024). Characterization of the small Arabidopsis thaliana GTPase and ADP-ribosylation factor-like 2 protein TITAN. J Cell Sci . 2024 Aug 14;137(15):jcs262315. doi: 10.1242/jcs.262315. (immunofluorescence)Suanno et al. (2023) Small extracellular vesicles released from germinated kiwi pollen (pollensomes) present characteristics similar to mammalian exosomes and carry a plant homolog of ALIX, Front. Plant Sci., 25 January 2023, Sec. Plant Membrane Traffic and Transport, Volume 14 - 2023.Chien et al. (2022) Phosphate transporter PHT1;1 is a key determinant of phosphorus acquisition in Arabidopsis natural accessions. Plant Physiol. 2022 Aug 29;190(1):682-697. doi: 10.1093/plphys/kiac250. PMID: 35639954; PMCID: PMC9434223. (Immunogold).Brumm, Singh, Kriechbaum, et al. (2022) N-terminal domain of ARF-GEF GNOM prevents heterodimerization with functionally divergent GNL1 in Arabidopsis. Plant J. 2022;112(3):772-785. doi:10.1111/tpj.15979.Farago et al. (2022) Small paraquat resistance proteins modulate paraquat and ABA responses and confer drought tolerance to overexpressing Arabidopsis plants. Plant Cell Environ. 2022 Jul;45(7):1985-2003. doi: 10.1111/pce.14338. Epub 2022 Apr 29. PMID: 35486392; PMCID: PMC9324991.Narasimhan et al. (2021) Systematic analysis of specific and nonspecific auxin effects on endocytosis and trafficking. Plant Physiol. 2021 Mar 18:kiab134. doi: 10.1093/plphys/kiab134. Epub ahead of print. PMID: 33734402.Hurny et al. (2020). SYNERGISTIC ON AUXIN AND CYTOKININ 1 Positively Regulates Growth and Attenuates Soil Pathogen Resistance. Nat Commun. 2020 May 1;11(1):2170. doi: 10.1038/s41467-020-15895-5. (immunolocalization)Kuang et al. (2019). Quantitative Proteome Analysis Reveals Changes in the Protein Landscape During Grape Berry Development With a Focus on Vacuolar Transport Proteins. Front Plant Sci. 2019 May 15;10:641. doi: 10.3389/fpls.2019.00641. eCollection 2019.Singh et al. (2018). A single class of ARF GTPase activated by several pathway-specific ARF-GEFs regulates essential membrane traffic in Arabidopsis. PLoS Genet. 2018 Nov 15;14(11):e1007795. doi: 10.1371/journal.pgen.1007795.Lang et al. (2011).Simultaneous isolation of pure and intact chloroplasts and mitochondria from moss as the basis for sub-cellular proteomics. Plant Cell Rep. 2011 Feb;30(2):205-15.doi: 10.1007/s00299-010-0935-4.
- References describing immunolocalization (IF) and (IG) studies:Pimpl et al (2000). In Situ Localization and in Vitro Induction of Plant COPI-Coated Vesicles. Plant Cell. 2000 Nov;12(11):2219-36.Ritzenthaler et al. (2002). Reevaluation of the Effects of Brefeldin A on Plant Cells Using Tobacco Bright Yellow 2 Cells Expressing Golgi-Targeted Green Fluorescent Protein and COPI Antisera. Plant Cell. 2002 Jan;14(1):237-61.
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