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{{/_source.additionalInfo}}Anti-Sar1 | Secretion-associated and Ras-related protein 1
(Cat#: AS08 326)
Description
- Immunogen: GST fusion of full length recombinant Sar1 of Arabidopsis thaliana UniProt: Q8VYP7, TAIR: At3g62560
- Host: Rabbit
- Clonality: Polyclonal
- Purity: Serum
- Format: Lyophilized
- Quantity: 100 µl
- Reconstitution: For reconstitution add 100 µl of sterile water
- Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
- Tested applications: Immunogold (IG), Western blot (WB)
- Recommended dilutions: 1 : 50 (IG), 1 : 500 (WB)
- Expected | apparent MW: 21 kDa (Arabidopsis thaliana)
- Confirmed reactivity: Arabidopsis thaliana, Echinochloa crus-galli Eragrostis neomexicana, Digitaria sanguinalis, Pteris vittata (fern), Panicum miliaceum, Panicum maximum, Petunia hybrida, Triticum aestivum, Zea mays
- Not reactive in: Nicotiana tabacum
- Sar1 belongs to a small GTPase superfamily and GTP binging activity. This protein is involved in intracellular protein transport. There are two different non-clathrin-coated vesicles that are responsible for transport between ER and the Golgi. Coat protein complexes involving membrane-associated GTP binging proteins – Arf1 and Sar1p for COPI and COPII are needed for formation of COP-coated vesicles. Sar1p is a cytosolic protein. It is temporarily recruited onto the membranes of the ER.
- Shen et al. (2014). The fronds tonoplast quantitative proteomic analysis in arsenic hyperaccumulator Pteris vittata L. J Proteomics. 2014 Feb 4. pii: S1874-3919(14)00047-5. doi: 10.1016/j.jprot.2014.01.029.Liu et al. (2014). SCFSLF-mediated cytosolic degradation of S-RNase is required for cross-pollen compatibility in S-RNase-based self-incompatibility in Petunia hybrida. Front. Genet., 22 July 2014 | doi: 10.3389/fgene.2014.00228
- Immunolocalization method with Sar1 antibodies is described in: Yao-dong Yang (2005). Dynamics of COPII vesicles and the Golgi apparatus in cultured Nicotiana tabacum BY-2 cells provides evidence for transient association of Golgi stacks with endoplasmic reticulum exit sites. Plant Cell. 2005 May;17(5):1513-31. Epub 2005 Apr 1.
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