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{{/_source.additionalInfo}}Anti-H3 | Histone H3 (rabbit antibody) (nuclear marker)
(Cat#: AS10 710)
Description
- Immunogen: KLH-conjugated synthetic peptide derived from known H3 sequences, inluding Arabidopsis thaliana H3.3 P59169 (At4g40030, At4g40040, At5g10980), H3.2 P59226 (At1g09200, At3g27360, At5g10390, At5g10400, At5g65360), H3-like 2 Q9FXI7 (At1g19890)
- Host: Rabbit
- Clonality: Polyclonal
- Purity: Serum
- Format: Lyophilized
- Quantity: 50 µl
- Reconstitution: For reconstitution add 50 µl of sterile water
- Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
- Tested applications: ChIp-qPCR (ChIp-qPCR), Immunocytochemistry (ICC), Immunofluorescence (IF), Western blot (WB)
- Recommended dilutions: 1 : 100-1 : 500 (ICC), 2 µl of antibody/500 µl solution (ChIp-qPCR), 1: 500 (IF), 1 : 5000 (WB)
- Expected | apparent MW: 15 | 17 kDa
- Confirmed reactivity: Arabidopsis thaliana, Botrytis cinerea, Brassica oleracea, Capsicum annuum, Cicer arietinum L., Chlamydomonas acidophila, Chlamydomonas reinhardtii, Cucumis sativus L cv Suyo, Cucurbita pepo L. var. cylindrica, Hordeum vulgare, human, Nicotiana benthamiana, Phalaenopsis equestris, Phaeodactylum tricornutum, Physcomitrium patens, Salicornia europaea, Solanum lycopersicum, Solanum sogarandinum, Solanum tuberosum, Vicia faba, Zea mays
- Not reactive in: No confirmed exceptions from predicted reactivity are currently known
- Histone 3 (H3) located in nuclei, incorporated into chromatin. Present in nucleosome together with H2A, H2B and H4.
- Martín-Merchán et al. (2024). Arabidopsis AGO1 N-terminal extension acts as an essential hub for PRMT5 interaction and post-translational modifications. Nucleic Acids Res . 2024 May 20:gkae387.doi: 10.1093/nar/gkae387. Mosesso et al. (2024). Arabidopsis CaLB1 undergoes phase separation with the ESCRT protein ALIX and modulates autophagosome maturation. Nat Commun. 2024 Jun 19;15(1):5188. doi: 10.1038/s41467-024-49485-6.Nguyen et al.(2024). The processed C-terminus of AvrRps4 effector suppresses plant immunity via targeting multiple WRKYs.J Integr Plant Biol. 2024 Jun 13.doi: 10.1111/jipb.13710. Kimura and Kanegae (2024).A phytochrome/phototropin chimeric photoreceptor promotes growth of fern gametophytes under limited light conditions. Exp Bot . 2024 Apr 15;75(8):2403-2416. Zhao et al. (2024). Psb28 protein is indispensable for stable accumulation of PSII core complexes in Arabidopsis.Plant J. 2024 May 26. doi: 10.1111/tpj.16844. Ciesielska et al. (2024). S2P2-the chloroplast-located intramembrane protease and its impact on the stoichiometry and functioning of the photosynthetic apparatus of A. thaliana. Front Plant Sci. 2024 Mar 15:15:1372318. doi: 10.3389/fpls.2024.1372318. Kim et al. (2024).Photoautotrophic cultivation of a Chlamydomonas reinhardtii mutant with zeaxanthin as the sole xanthophyll. Biotechnol Biofuels Bioprod. 2024 Mar 14;17(1):41. doi: 10.1186/s13068-024-02483-8. Woo et al.(2024). Attenuation of phytofungal pathogenicity of Ascomycota by autophagy modulators. Nat Commun. 2024 Feb 29;15(1):1621.doi: 10.1038/s41467-024-45839-2. Liu et al. (2023). RBPome identification in egg-cell like callus of Arabidopsis. Biol Chem. 2023 Sep 29;404(11-12):1137-1149.doi: 10.1515/hsz-2023-0195. Hyun et al. (2023). Functional demonstration of Aureochrome 1a proteasomal degradation after blue light incubation in the diatom Phaeodactylum tricornutum. J Plant Physiol. 2023 Dec 1:292:154148. doi: 10.1016/j.jplph.2023.154148 Skalický et al. (2023). Fluorescence-activated multi-organelle mapping of subcellular plant hormone distribution. Plant J. 2023 Dec;116(6):1825-1841.doi: 10.1111/tpj.16456. Epub 2023 Sep 8. Farago et al. (2022) Small paraquat resistance proteins modulate paraquat and ABA responses and confer drought tolerance to overexpressing Arabidopsis plants. Plant Cell Environ. 2022 Jul;45(7):1985-2003. doi: 10.1111/pce.14338. Epub 2022 Apr 29. PMID: 35486392; PMCID: PMC9324991.Margaritopoulou et al (2021) Enriched HeK4me3 marks at Pm-0 resistance-related genes prime courgette against Podosphaera xanthii. Plant Physiol. 2021 Sep 21:kiab453. doi: 10.1093/plphys/kiab453. Epub ahead of print. Erratum in: Plant Physiol. 2021 Nov 11;: PMID: 34597395.
- Specific fluorescence in ICC has been observed for interphase nuclei as well as around centromer region (where Ser10 of histone H3 is phosphorylated) in mitotic chromosomes
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