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{{/_source.additionalInfo}}Anti-NPR1 | Nonexpresser of PR genes 1
(Cat#: AS12 1854)
Description
- Immunogen: KLH-conjugated peptide, chosen from NPR1 sequence of Arabidopsis thaliana, TAIR: AT1G64280, UniProt: P93002
- Host: Rabbit
- Clonality: Polyclonal
- Purity: Immunogen affinity purified serum in PBS pH 7.4.
- Format: Lyophilized
- Quantity: 50 µg
- Reconstitution: For reconstitution add 50 µl of sterile water
- Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
- Tested applications: Western blot (WB)
- Recommended dilutions: 1 : 1000 (WB)
- Expected | apparent MW: 66 | 66 kDa
- Confirmed reactivity: Arabidopsis thaliana
- Not reactive in: Marchantia sp., Nicotiana benthamiana, Nicotiana tabacum, Solanum lycopersicum, Solanum tuberosum
- NPR1 (regulatory protein NPR1) is a key positive regulator of the SA-dependent signaling pathway that negatively regulates JA-dependent signaling pathway. Controls the onset of systemic acquired resistance (SAR). Subcellular localization: cytoplasm, nucleaus (following induction by salicylic acid treatment or after pathogen infection. Alternative names: BTB/POZ domain-containing protein NPR1, Non-inducible immunity protein 1, Nim1, Salicylic acid insensitive 1, Sai1, ATNPR1.
- Yao et al. (2023). High air humidity dampens salicylic acid pathway and NPR1 function to promote plant disease.EMBO J. 2023 Nov 2;42(21):e113499.doi: 10.15252/embj.2023113499. Arenas-Alfonseca et al. (2021) Arabidopsis beta-cyanoalanine synthase mutation overcomes NADPH oxidase action in response to pathogens. J Exp Bot. 2021 Mar 26:erab137. doi: 10.1093/jxb/erab137. Epub ahead of print. PMID: 33770168.Nomoto et al. (2021) Suppression of MYC transcription activators by the immune cofactor NPR1 fine-tunes plant immune responses. Cell Rep. 2021 Dec 14;37(11):110125. doi: 10.1016/j.celrep.2021.110125. PMID: 34910911.Lei et al. (2020). Construction of gold-siRNANPR1 nanoparticles for effective and quick silencing of NPR1 in Arabidopsis thaliana. DOI: 10.1039/D0RA02156C (Paper) RSC Adv., 2020, 10, 19300-19308
- Please note that depending upon detection system you are using, longer exposure time may be required with this antibody. Chemiluminescent detection reagent in extreme femtogram range is necessary.
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