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{{/_source.additionalInfo}}Anti-HA (rabbit polyclonal)
(Cat#: AS12 2220)
Description
- Immunogen: KLH-conjugated synthetic peptide: YPYDVPDYA
- Host: Rabbit
- Clonality: Polyclonal
- Purity: affinity purified serum in PBS, pH 7.4.
- Format: Lyophilized
- Quantity: 100 µg
- Reconstitution: For reconstitution add 100 µl of sterile water
- Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
- Tested applications: Western blot (WB), immunoprecipitation (IP)
- Recommended dilutions: 1 : 5000 (WB)
- Expected | apparent MW: Depends on the MW of the protein which is HA-tagged
- Confirmed reactivity: HA-tag
- Not reactive in: No confirmed exceptions from predicted reactivity are currently known
- HA-tag is derived from a human influenza hemagglutinin HA-molecule corresponding to amino acids 96-106 and is used as a general epitope tag in expression vectors.
- Tanigawa et al. (2024). FYVE1/FREE1 is involved in glutamine-responsive TORC1 activation in plants. iScience. 2024 Aug 26;27(9):110814. doi: 10.1016/j.isci.2024.110814. Hwang et al. (2019) Arabidopsis ABF3 and ABF4 Transcription Factors Act with the NF-YC Complex to Regulate SOC1 Expression and Mediate Drought-Accelerated Flowering. Mol Plant. 2019 Apr 1;12(4):489-505. doi: 10.1016/j.molp.2019.01.002
- Important note: there is a strong cross reactivity between 25 -20 kDa in Arabidopsis thaliana wildtype.
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