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{{/_source.additionalInfo}}Anti-Homogalacturonan-1 (clone CCRC-M38)
(Cat#: AS16 3117-1ml)
Description
- Immunogen: MeBSA-conjugated Arabidopsis thaliana seed mucillage (Hemogalacturonan), non-covalent, Epitope structure for crabohydrate antigen: de-estrified homogalacturonan (DP>4),
- Host: Mouse
- Clonality: Monoclonal
- Purity: Cell culture supernatant.
- Format: Liquid
- Quantity: 1 ml
- Storage: Antibody can be stored up to 1 month at 4°C, and over 1 month at -80°C. Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
- Tested applications: ELISA (ELISA), Immunohistochemistry (IHC), Immunofluorescence (IF)
- Recommended dilutions: 1:10 (IHC)
- Confirmed reactivity: Arabidopsis thaliana
- Not reactive in: No confirmed exceptions from predicted reactivity are currently known
- Homogalacturonan (HG) is a pectic polysaccharide, composed of α-1,4 linked galacturonic acid residues. Pectin consists of complex net of polysacchari.des which are found in the primary cell walls of terrestial plants and are enriched in non-woody parts. Pectin acts as an extracellular glue which binds cells together.
- Pattathil et al. (2012). Immunological approaches to plant cell wall and biomass characterization: Glycome Profiling. Methods Mol Biol. 2012;908:61-72.doi: 0.1007/978-1-61779-956-3_6. Patathil et al. (2010). A comprehensive toolkit of plant cell wall glycan-directed monoclonal antibodies. Plant Physiol. 2010 Jun;153(2):514-25.doi: 10.1104/pp.109.151985.
- This antibody binds to a de-esterified ?-1,4 linked homogalcturonan epitope with a degree of polymerizaion of 4 or more, It does not bind to a homogalacturonan trimer
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