Anti-H3K18me2 | Histone H3 (dimethylated Lys18)

(Cat#: AS16 3182)
Description
  • Immunogen: KLH-conjugated synthetic peptide
  • Host: Rabbit
  • Clonality: Polyclonal
  • Purity: Immunogen affinity purified serum.
  • Format: Liquid
  • Quantity: 50 µg
  • Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
  • Tested applications: Chromatin immunoprecipitation (ChIP), Dot blot (Dot), Immunofluorescence (IF), Western blot (WB)
  • Recommended dilutions: 2-5 µg/million cells (ChIP), 1 : 1000 (Dot), 1 : 500 (IF), 1 : 100 (IHC), 1 : 500 (WB)
  • Expected | apparent MW: 15 kDa
  • Confirmed reactivity: Human
  • Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • The di-methylated K18 on histone H3 is a seemingly transient post-translational modification. H3K18 is better known to be acetylated, and occasionally mono-methylated. Suv39h1, a well-studied histone methyltransferase seems to be responsible for the transition of acetylation and methylation at this H3 modification site. The di-methylated K18 on H3 seems to be associated with embryological development and possibly implantation.

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