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{{/_source.additionalInfo}}Anti-NAD-ME | Mitochondrial NAD-dependent malic enzyme
(Cat#: AS16 3932)
Description
- Immunogen: Native, NAD-ME purified from mitochondria isolated from mature amaranth leaves, UniProt: P37224
- Host: Rabbit
- Clonality: Polyclonal
- Purity: Serum + 0.01% sodium azide.
- Format: Lyophilized
- Quantity: 50 µl
- Reconstitution: For reconstitution add 50 µl of sterile water
- Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
- Tested applications: Immunoprecipiatation (IP), Immunolocalization (IL), Western blot (WB)
- Recommended dilutions: 1 : 25 to 1 : 100 (IL), 1 : 1000 (WB)
- Expected | apparent MW: 65 kDa
- Confirmed reactivity: Amaranthus hypochondriacus, Arabidopsis thaliana, Flaveria sp., Glycine max, Nicotiana tabacum, Zea mays
- Not reactive in: No confirmed exceptions from predicted reactivity are currently known
- NAD-ME (Mitochondrial NAD-dependent malic enzyme) is an enzyme which in some C4 plants catalyzes the decarboxylation of 4 carbon malate in the bundle sheath cells, releasing carbon dioxide. This enzyme plays a key role in photosynthetic carbon fixation.
- Hoa et al. (2024). Proteomic analysis on symbiotic differentiation of mitochondria in soybean nodules. Comparative Study Plant Cell Physiol. 2004 Mar;45(3):300-8. doi: 10.1093/pcp/pch035.Long et al. (1994). Cloning and analysis of the C4 photosynthetic NAD-dependent malic enzyme of amaranth mitochondria. J Biol Chem. 1994 Jan 28;269(4):2827-33.
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