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{{/_source.additionalInfo}}Anti-PA200 | Proteasome activator PA200
(Cat#: AS19 4269)
Description
- Immunogen: PA200 antibodies were generated against a partial fragment encompassing residues 840-1140 Arabidopsis thaliana protein sequence UniProt:F4JC97-1, TAIR: At3g13330
- Host: Rabbit
- Clonality: Polyclonal
- Purity: Serum
- Format: Lyophilized
- Quantity: 50 µl
- Reconstitution: For reconstitution add 50 µl, of sterile water
- Storage: Store lyophilized/reconstituted at -20°C (short tem, months) or at -80°C (long term, years) ; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
- Tested applications: Western blot (WB)
- Recommended dilutions: 1 : 500 (WB)
- Expected | apparent MW: 202.9 | ca. 200 kDa
- Confirmed reactivity: Arabidopsis thaliana
- Not reactive in: No confirmed exceptions from predicted reactivity are currently known
- PA200 (Proteasome activator PA200) is an associated component of the proteasome that specifically recognizes acetylated histones and promotes ATP- and ubiquitin-independent degradation of core histones during DNA damage response. It also recognizes and binds acetylated histones via its bromodomain-like (BRDL) region and activates the proteasome by opening the gated channel for substrate entry. Binds to the core proteasome via its C-terminus, which occupies the same binding sites as the proteasomal ATPases, opening the closed structure of the proteasome via an active gating mechanism. involved in DNA damage response: binds to acetylated histones and promotes degradation of histones. Protein levels increase upon exposure of seedlings to MG132, a specific, potent, reversible, and cell-permeable proteasome inhibitor. Alternative names: Proteasome activator subunit 4, Proteasome activating protein 200.
- Book et al. (2010). Affinity purification of the Arabidopsis 26 S proteasome reveals a diverse array of plant proteolytic complexes. J Biol Chem. 2010 Aug 13;285(33):25554-69. doi: 10.1074/jbc.M110.136622.
- Recommended protein load is 20 µg/well, primary antibody dilution: 1: 1000 ON/4°C and optimisation based on obtained result regarding signal/noise ratio,
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