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{{/_source.additionalInfo}}Anti-WHIRLY2
(Cat#: AS16 3952)
Description
- Immunogen: KLH-conjugated peptide derived from HvWHIRLY2 protein sequence, NCBI
- Host: Chicken
- Clonality: Polyclonal
- Purity: Immunogen affinity purified IgY in PBS pH 8 with 0.02 % sodium azide.
- Format: Liquid
- Quantity: 50 µg
- Storage: Store at 4°C; make aliquots to avoid working with a stock. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
- Tested applications: Immunogold (IG), Western blot (WB)
- Recommended dilutions: 1: 100 (IG), 1: 1500 (WB)
- Expected | apparent MW: 25 kDa
- Confirmed reactivity: Hordeum vulgare
- Not reactive in: Glycine max
- WHIRLY proteins are multifunctional DNA/RNA-binding proteins (Prikryl et al. 2008, NAR 36: 5152-5165). WHIRLY1 was shown to be located both in chloroplasts and nucleus (Grabowski et al. 2008, Plant Physiology 147:1800-1804). In chloroplasts it is part of nucleoids (Pfalz et al. 2006, Plant Cell 18:176-197; Melonek et al. 2010, Planta 232:471-481). In the nucleus, WHIRLY1 is an acitivator of pathogen response genes (Desveaux et al. 2000, Plant Cell 12:1477-1489). WHIRLY2 is a mitochondrial protein and affects DNA copy number (Cai et al. 2015, Plant Physiology 169:660-673).
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