Anti-WHIRLY2

(Cat#: AS16 3952)
AS16_3952
AS16_3952_1
Description
  • Immunogen: KLH-conjugated peptide derived from HvWHIRLY2 protein sequence, NCBI
  • Host: Chicken
  • Clonality: Polyclonal
  • Purity: Immunogen affinity purified IgY in PBS pH 8 with 0.02 % sodium azide.
  • Format: Liquid
  • Quantity: 50 µg
  • Storage: Store at 4°C; make aliquots to avoid working with a stock. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
  • Tested applications: Immunogold (IG), Western blot (WB)
  • Recommended dilutions: 1: 100 (IG), 1: 1500 (WB)
  • Expected | apparent MW: 25 kDa
  • Confirmed reactivity: Hordeum vulgare
  • Not reactive in: Glycine max
  • WHIRLY proteins are multifunctional DNA/RNA-binding proteins (Prikryl et al. 2008, NAR 36: 5152-5165). WHIRLY1 was shown to be located both in chloroplasts and nucleus (Grabowski et al. 2008, Plant Physiology 147:1800-1804). In chloroplasts it is part of nucleoids (Pfalz et al. 2006, Plant Cell 18:176-197; Melonek et al. 2010, Planta 232:471-481). In the nucleus, WHIRLY1 is an acitivator of pathogen response genes (Desveaux et al. 2000, Plant Cell 12:1477-1489). WHIRLY2 is a mitochondrial protein and affects DNA copy number (Cai et al. 2015, Plant Physiology 169:660-673).

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