Anti-Tubulin beta chain (monoclonal antibody)

(Cat#: AS20 4484)
AS20_4484
AS20_4484_1
Description
  • Immunogen: Beta-tubulin purified from porcine brain, UniProt: Q9H4B7
  • Host: Mouse
  • Clonality: Monoclonal
  • Purity: Purified by precipitation and affinitychromatography in TBS. Contains 15 mM sodium azide.
  • Format: Liquid
  • Quantity: 100 µg
  • Storage: Store at 4°C; Do not freeze. Do not exceed exipry date is provided on the tube. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
  • Tested applications: Immunocytochemistry (ICC), Immunohistochemistry on frozen sections (IHC), Western blot (WB)
  • Recommended dilutions: 2-8 µg/ml (ICC), 1 µg/ml (WB)
  • Expected | apparent MW: 50.3 kDa
  • Confirmed reactivity: Human, mouse, pig
  • Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Tubulin beta (TUB) together with alpha tubulin is making up microtubules.
  • This antibody is recognizing N-terminal structural domain of beta tubulin.Recommended secondary antibody for Western blot is: goat anti mouse IgM AS16 3497Metal induced stress affected the expression of tubulin alpha and gamma, and that therefore, these proteins cannot be used as a loading control under that type of conditions. More information can be found here.

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