CRISPRa dCas9-VPH Expression Construct

CRISPRa dCas9-VPH pRDVCCBla-CMV-dCas9-VPH-2A-Blast (plasmid), 25 ug (Cat# SVVPHC9B-PS)
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Sales Price$750.00
Format 
Size 
Vector 
Description

 These ready-to-use dCas9-VPH expressing constructs ensure optimal expression for transcriptional activation. The constructs only contain one selection marker, either antibiotic-resistant gene or fluorescent protein. Multiple promoter options are available enabling flexible CRISPRa applications across a range of cell types. 

Available formats include 6 CRISPRa dCas9-VPH vector configurations, offered as either 25 µg plasmid DNA or pre-packaged lentiviral particles at 1 × 10^6 TU. Vector options include constitutive dCas9-VPH expression driven by CMV, EFS, RSV, or SFFV promoters with selectable markers (Blast, Puro) or a TagGFP2 fluorescent reporter. 

  • Expressing the dCas9-VPH complex
  • 6dCas9-VPH vectors
  • CMV, EFS, RSV, or SFFV promotor options
  • CRISPR Knockout Screens
  • Functional Genetic Screen

Cat #

Vector

Format

Size

SVVPHC9B-PS

CRISPRa dCas9-VPH pRDVCCBla-CMV-dCas9-VPH-2A-Blast

Plasmid

25 ug

SVVPHC9B-VS CRISPRa dCas9-VPH pRDVCCBla-CMV-dCas9-VPH-2A-Blast 

Virus

1 x 10^6 TU

SVVPHC9E2B-PS CRISPRa dCas9-VPH pRDVCE2Bla-EFS-dCas9-VPH-2A-Blast (plasmid)

Plasmid

25 ug

SVVPHC9E2B-VS CRISPRa dCas9-VPH pRDVCE2Bla-EFS-dCas9-VPH-2A-Blast (virus)

Virus

1 x 10^6 TU

SVVPHC9G-PS CRISPRa dCas9-VPH pR-CMV-dCas9-VPH-2A-TagGFP2 (plasmid)

Plasmid

25 ug

SVVPHC9G-VS CRISPRa dCas9-VPH pR-CMV-dCas9-VPH-2A-TagGFP2 (virus)

Virus

1 x 10^6 TU

SVVPHC9P-PS CRISPRa dCas9-VPH pRDVCCP-CMV-dCas9-VPH-2A-Puro (plasmid)

Plasmid 

25 ug

SVVPHC9P-VS CRISPRa dCas9-VPH pRDVCCP-CMV-dCas9-VPH-2A-Puro (virus)

Virus

1 x 10^6 TU

SVVPHC9RB-PS CRISPRa dCas9-VPH pRDVCRBla-RSV-dCas9-VPH-2A-Blast (plasmid)

Plasmid

25 ug

SVVPHC9RB-VS CRISPRa dCas9-VPH pRDVCRBla-RSV-dCas9-VPH-2A-Blast (virus) Virus 1 x 10^6 TU
SVVPHC9SFB-PS CRISPRa dCas9-VPH pRDVCSFBla-SFFV-dCas9-VPH-2A-Blast (plasmid) Plasmid 25 ug
SVVPHC9SFB-VS CRISPRa dCas9-VPH pRDVCSFBla-SFFV-dCas9-VPH-2A-Blast (virus) Virus 1 x 10^6 TU

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