DriverMap AIR-DNA Assay

DriverMap™ AIR-DNA CDR3 Profiling Assays

Cellecta's DriverMap™ AIR-DNA Assays provide sensitive, comprehensive profiling of T-cell receptor (TCR) and B-cell receptor (BCR) CDR3 repertoires from genomic DNA. Using highly multiplexed PCR amplification and next-generation sequencing (NGS), these assays enable researchers to identify and quantify immune receptor clonotypes, assess repertoire diversity, and investigate clonal expansion in T- and B-cell populations.

The assays utilize 315 forward primers targeting the FR3 variable region and 100 reverse primers targeting the J region of seven immune receptor genes. TCR (TRA, TRB, TRD, TRG) and BCR (IGH, IGK, IGL) repertoires are amplified in separate reactions to support balanced profiling. Cellecta's proprietary Validator Barcode (VBC) technology incorporates internal barcoded replicates to improve clonotype quantification and help identify amplification and sequencing errors.

DNA-based profiling provides a more direct measure of clonotype-associated cell abundance than RNA-based profiling and is particularly useful for studies requiring quantitative analysis of clonal populations. The stability of genomic DNA also makes these assays well suited for challenging sample types, including FFPE tissues and tumor biopsies.