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{{/_source.additionalInfo}}Universal Unique Dual Index (UDI) NGS Plates
Unique Dual Index Set A for DriverMap EXP or AIR (1-96) (Cat#DM-NGS-SetA-B2)
Description
Universal Unique Dual Index (UDI) NGS Plates
These Universal Unique Dual Index (UDI) NGS Plates provide the indexing primers required for next-generation sequencing (NGS) libraries prepared using DriverMap™ Adaptive Immune Receptor (AIR) Profiling and DriverMap™ Targeted RNA-Seq Expression (EXP) Reagent Kits. These separately purchased plates enable accurate sample identification and demultiplexing when multiple sequencing libraries are pooled for analysis on compatible Illumina® NGS platforms.
Each 96-well plate contains 96 unique dual-index combinations, allowing individual sequencing libraries to be distinguished during multiplexed sequencing. The use of unique index pairs helps reduce the risk of incorrect sample assignment associated with index hopping and improves the reliability of sequencing data.
The UDI plates are available in four distinct, non-overlapping index sets (A, B, C, and D), providing a total of 384 unique dual-index combinations when all four sets are used. Each well contains 20 µL of 10X concentrated index primer mix, sufficient for up to four indexing reactions per well.
The plates are compatible with DriverMap AIR and EXP workflows, including applicable bulk immune receptor profiling, single-cell immune receptor profiling, and targeted gene expression assays.
- Unique Dual Indexing: Provides 96 distinct dual-index combinations per plate for sample identification and demultiplexing.
- Four Non-Overlapping Index Sets: Sets A, B, C, and D collectively provide 384 unique index combinations for higher-throughput multiplexing.
- Improved Sample Assignment: Unique index pairs help reduce incorrect sample assignments associated with index hopping during multiplexed NGS.
- Concentrated Primer Mix: Each well contains 20 µL of 10X concentrated index primer mix.
- Multiple Reactions per Well: Sufficient primer mix for up to four indexing reactions per well, or 384 reactions per plate when used according to the recommended protocol.
- DriverMap Compatibility: Designed for use with compatible DriverMap AIR and EXP profiling reagent kits.
- Indexing NGS libraries prepared using DriverMap AIR TCR/BCR Profiling Assays
- Indexing libraries prepared using DriverMap Single-Cell AIR (scAIR) Assays
- Indexing libraries prepared using DriverMap scAIR Immunophenotyping Assays
- Indexing libraries prepared using DriverMap EXP Targeted RNA-Seq Expression Profiling Kits
- Reducing incorrect sample assignment associated with index hopping
- Multiplexed sequencing and sample demultiplexing on compatible Illumina NGS platforms
|
Cat # |
Description |
Size |
|
DM-NGS-SetA-B2 |
Unique Dual Index Set A for DriverMap EXP or AIR (1–96) |
1 × 96-well plate (4 rxn/well) |
|
DM-NGS-SetB-B2 |
Unique Dual Index Set B for DriverMap EXP or AIR (97–192) |
1 × 96-well plate (4 rxn/well) |
|
DM-NGS-SetC-B2 |
Unique Dual Index Set C for DriverMap EXP or AIR (193–288) |
1 × 96-well plate (4 rxn/well) |
|
DM-NGS-SetD-B2 |
Unique Dual Index Set D for DriverMap EXP or AIR (289–384) |
1 × 96-well plate (4 rxn/well) |
Related Products
DriverMap AIR-DNA Assay
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CRISPR Cas9- and dCas9-Expressing Cell LinesBoca Scientific is your premiere source for high-quality, innovative solutions for Cell Biology, Molecular Biology, Immunology, genetics and other lab products and reagents. We bring leading-edge products from our own-line and around the world to laboratories in the US and Canada. Our goal is to offer excellent solutions to drive research and discoveries backed by superior customer support.
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