Fast Bst RT Mix

100 rxn (Cat #8601S)
8601M
Sales Price$340.00
Size 
Description

Fast Bst RT Mix is a ready-to-use mix containing recombinant DNA polymerase expressed by Geobacillus stearothermophilus. The Bst DNA polymerase displays high strand displacement activity, exhibits 5’ to 3’ polymerase activity, but lacks 5’ to 3’ exonuclease activity. Fast Bst RT Mix also contains an efficient thermostable reverse transcriptase with an RNase inhibitor (RTase Amp) to prevent degradation of RNA templates by RNases. Fast Bst RT Mix is tolerant to inhibitors, enabling rapid and robust isothermal nucleic acid amplification reactions at a constant temperature. The typical reaction temperature is 65°C. Addition of an intercalating dye allows the reaction to be monitored using a real-time PCR instrument

 

  • Ready-to-use
  • High strand displacement activity in an optimized formulation
  • Rapid and robust LAMP reactions at a constant temperature
  • Prevents degradation of RNA templates by RNase allowing simultaneous reverse transcription and amplification of RNA target templates
  • Compatible with real-time PCR instruments and also can be used with small and portable instruments
  • Isothermal amplification
  • Isothermal LAMP
  • Real-time detection
  • #8601S - 100 rxn:  1 x 1.25 ml 2x Fast Bst Mix; 1 x 0.2 ml RTase Amp; 1 x 0.125 ml 20x Fluorescent dye
  • #8601M - 500 rxn:  4 x 1.6 ml 2x Fast Bst Mix; 1 x 1 ml RTase Amp; 1 x 0.625 ml 20x Fluorescent dye
 

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