HiDi® Taq 2x PCR Master Mix

HiDi® stands for High Discrimination of mismatches at the 3’-terminus of primers in PCR. HiDi® Taq 2x PCR Master Mix is a ready-to-use master mix specially optimized for assays in which high discrimination is required. The combination of highly selective aptamer-based fast-start formulated HiDi® Taq DNA polymerase and an optimized buffer with ultrapure dNTPs ensures simple reaction setup and reliable results. HiDi® Taq DNA polymerase efficiently amplifies from primers that are matched at the 3'-end and discriminates primers that are mismatched. The variant has a 5'-3'-exonuclease activity and therefore, can be used for hydrolysis probe-based real-time PCRs. By using HiDi® Taq DNA polymerase, less than 10 copies of a mutation can be detected in a background of >10^4 wild-type copies.

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