CRISPR KO (CRISPR Knockout) Lentiviral sgRNA Libraries

The Cellecta product line offers high-quality pooled lentiviral CRISPR sgRNA libraries for unbiased phenotypic knockout screens in mammalian cell systems. Pooled libraries of many thousands of defined single-guide RNA (sgRNA or gRNA) sequences enable disruption (or “knockout”) of thousands of genes throughout a cell population in a single experiment. Screening this cell population for a specific phenotype (e.g., viability) then enables the identification of specific genes driving that phenotype, such as a drug response or disease phenotype.

Effective guides incorporate Cellecta’s tracrRNA “HEAT / HECG” design and have been screened to minimize off-target knockout.
Both plasmid and pre-packaged lentivirus forms of the libraries are available.
A sufficient quantity of the plasmid library is provided for immediate packaging (no re-amplification necessary).
Pooled CRISPRa and CRISPRi libraries are also available to study gene activation or inhibition.

Pooled CRISPR screens enable the identification of genes that control phenotypes such as viability, growth, or pathway activation using NGS-based readouts.

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