shRNA Pooled Knockdown Libraries

The Cellecta product line offers pre-made and custom pooled shRNA libraries that have been used in a number of highly cited and published genetic screening experiments for discovery and functional characterization of novel therapeutic targets.

• Easily obtain proven high quality, highly cited pooled shRNA libraries
• Confidently screen for genes functionally required for drug mechanisms of action, disease progression or other biological responses
• Embedded shRNA barcodes eliminate the need to sequence hairpins

The pre-made pooled and custom lentiviral-based libraries containing heterogeneous mixtures of shRNA that allow you to assay the effects of many thousands of constructs in one experiment. Our pre-made, pooled lentiviral genome-wide shRNA libraries target nearly all protein-encoding genes for human or mouse. In addition, we can make precisely defined high quality custom pooled libraries with well designed shRNAs targeting any set of your genes of interest in just a couple of months.

By taking advantage of array-based oligonucleotide synthesis technologies, we can readily make precisely defined large custom pooled libraries expressing many thousands of shRNAs. In approximately 2 months, we can produce a completely new, high-quality custom library focusing on any target set of genes. The library vector can also be easily customized, and the same library oligonucleotides can be cloned into more than one vector backbone (e.g. with an inducible and constitutive shRNA). It is a very flexible platform that enables us to easily meet your experimental needs.

Screening readout of enrichment or depletion representation of particular shRNAs is greatly facilitated by the incorporation of easily sequenced unambiguous barcodes in each shRNA construct. Direct amplification and sequencing of hairpins is problematic, and increases variability in the data since small sequence variations of the hairpin stem significantly affect the efficiency of PCR leading to increased noise in the data readout. The incorporation of shRNA-specific barcodes addresses this problem inherent to pooled shRNA screens.

Citations:

• Toll-like receptor 4 signaling activates ERG function in prostate cancer and provides a therapeutic target (2021)
• Relaxed initiation pausing of ribosomes drives oncogenic translation (2021)
• Identification of genetic targets in acute myeloid leukaemia for designing targeted therapy (2020)
• Genome-wide screening reveals a role for subcellular localization of CRBN (2020)
• Histone Acetyltransferase p300 Induces De Novo Super-Enhancers (2019)