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{{/_source.additionalInfo}}Anti-Clathrin heavy-chain 1,2
(Cat#: AS10 690)
Description
- Immunogen: KLH-conjugated peptide derived from available plant clathrin heavy chain sequences including Arabidopsis thaliana clathrin heavy chain 1 UniProt: Q0WNJ6, TAIR:At3g11130, clathrin heavy chain 2 UniProt: Q0WLB5,TAIR:At3g08530
- Host: Rabbit
- Clonality: Polyclonal
- Purity: Immunogen affinity purified serum in PBS pH 7.4.
- Format: Lyophilized
- Quantity: 50 µg
- Reconstitution: For reconstitution add 50 µl of sterile water
- Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
- Tested applications: Immunolocalization (IL), Immunofluorescence (IF), Western blot (WB)
- Recommended dilutions: 1 : 2400 (IL), 1 :400 (IF), 1 : 2000 (WB)
- Expected | apparent MW: 193 | 170 kDa (Arabidopsis thaliana)
- Confirmed reactivity: Actinidia chinensis, Arabidopsis thaliana, Chlamydomonas reinhardtii, Nicotiana tabacumAS07 260
- Not reactive in: Nicotiana benthamiana
- Clathrin is a protein involved in intracellular trafficking and plays a major role in the formation of coated vesicles. It consists of three clathrin heavy chains and three light chains. Clathrin-coated vesicles (CCV) selectively sort cargo at the cell membrane, trans-Golgi network, and endosomal compartments for multiple membrane traffic pathways.
- Takáč et al. (2024). Actin cytoskeleton and plasma membrane aquaporins are involved in different drought response of Arabidopsis rhd2 and der1 root hair mutants. Plant Physiology and Biochemistry Available online 19 September 2024, 109137.Pan et al. (2023). Stress-induced endocytosis from chloroplast inner envelope membrane is mediated by CHLOROPLAST VESICULATION but inhibited by GAPC.Cell Rep. 2023 Oct 31;42(10):113208. doi: 10.1016/j.celrep.2023.113208.Fujimoto et al. (2020) Longin R-SNARE is retrieved from the plasma membrane by ANTH domain-containing proteins in Arabidopsis. Proc Natl Acad Sci U S A. 2020 Oct 6;117(40):25150-25158. doi: 10.1073/pnas.2011152117. Epub 2020 Sep 23. PMID: 32968023; PMCID: PMC7547277.Ranjan et al. (2019). Transient Internalization and Microtubule-Dependent Trafficking of a Ciliary Signaling Receptor from the Plasma Membrane to the Cilium. Curr Biol. 2019 Aug 2. pii: S0960-9822(19)30867-X. doi: 10.1016/j.cub.2019.07.022.Wattelet-Boyer et al. (2016). Enrichment of hydroxylated C24- and C26-acyl- chain sphingolipids mediates PIN2 apical sorting at trans-Golgi network subdomains. Nat Commun. 2016 Sep 29;7:12788. doi: 10.1038/ncomms12788.Derbyshire et al. (2015). Proteomic Analysis of Microtubule Interacting Proteins over the Course of Xylem Tracheary Element Formation in Arabidopsis. Plant Cell. 2015 Oct 2. pii: tpc.15.00314.Grones et al. (2015). Auxin-binding pocket of ABP1 is crucial for its gain-of-function cellular and developmental roles. J Exp Bot. 2015 Apr 28. pii: erv177.
- Please, note that fresh samples will provide better restuls (see image below)
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