Anti-Clathrin heavy-chain 1,2, Biotin-conjugated (40 µg)

(Cat#: AS10 690B)
AS10_690B
AS10_690B_1
Description
  • Immunogen: KLH-conjugated peptide derived from available plant clathrin heavy chain sequences including Arabidopsis thaliana clathrin heavy chain 1 UniProt: Q0WNJ6, TAIR:At3g11130, clathrin heavy chain 2 UniProt: Q0WLB5,TAIR:At3g08530
  • Host: Rabbit
  • Clonality: Polyclonal
  • Purity: Immunogen affinity purified serum in PBS pH 7.4, conjugated to biotin.
  • Format: Liquid
  • Quantity: 40 µg
  • Storage: Store at 4°C for 12-18 months. A preservative may be added for long time storage up to 2 years.
  • Tested applications: Immunolocalization (IL), Immunofluorescence (IF), Western blot (WB)
  • Recommended dilutions: 1 : 2400 (IL), 1 :400 (IF), 1 : 2000 (WB)
  • Expected | apparent MW: 193 | 170 kDa (Arabidopsis thaliana)
  • Confirmed reactivity: Arabidopsis thaliana, Chlamydomonas reinhardtii, Nicotiana tabacum
  • Not reactive in: Nicotiana benthamiana
  • Clathrin is a protein involved in intracellular trafficking and plays a major role in the formation of coated vesicles. It consists of three clathrin heavy chains and three light chains. Clathrin-coated vesicles (CCV) selectively sort cargo at the cell membrane, trans-Golgi network, and endosomal compartments for multiple membrane traffic pathways.
  • Please, note that fresh samples will provide better restuls (see image below)

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