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{{/_source.additionalInfo}}Anti-DCL3 | Dicer-like protein 3
(Cat#: AS12 2103)
Description
- Immunogen: KLH-conjugated synthetic peptide derived from Arabidopsis thaliana DCL3 sequence, Uniprot: Q9LXW7 , TAIR: At3g43920
- Host: Rabbit
- Clonality: Polyclonal
- Purity: Immunogen affinity purified serum in PBS pH 7.4.
- Format: Lyophilized
- Quantity: 200 µg
- Reconstitution: For reconstitution add 200 µl of sterile water
- Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
- Tested applications: Western blot (WB)
- Recommended dilutions: 1 : 1000 (WB)
- Expected | apparent MW: 117 kDa
- Confirmed reactivity: Arabidopsis thaliana
- Not reactive in: Zea mays
- DCL3 (EC=3.1.26) is a ribonuclease (RNase) III involved in RNA-mediated post-transcriptional gene silencing (PTGS). Functions in the microRNAs (miRNAs) biogenesis pathway by cleaving primary miRNAs (pri-miRNAs) and precursor miRNAs (pre-miRNAs). Synonymes: Endoribonuclease Dicer homolog 3.
- Recommend protein load is from 40-50 µg/well (using 1,5 mm spacers helps to obtain wider wells)
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