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Human Genome-Wide CRISPRi Dual-sgRNA Libary

Human Genome-Wide CRISPRi Dual-sgRNA Library (plasmid), 200ug (Cat# KIDHGW-105K-P)
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Sales Price$13,500.00
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Description

This off-the-shelf, ready-to-use human genome-wide sgRNA library is designed to inactivate or inhibit gene expression. The CRISPRi sgRNA Library targets nearly 19,000 human protein-coding genes, with 5 sgRNAs per gene. The library uses thesgRNAs in dual combinations across 5 constructs (for example, sgRNA 1 & 2, sgRNA 1 & 3, sgRNA 2 & 3, etc.). Unlike a CRISPR knockout, the induction is reversible since it does not alter the underlying DNA structure.  This library provides a tool for identifying candidate therapeutic targets involved in the regulation of growth, differentiation, disease pathogenesis, and other biological responses. Available formats include 200 ug plasmid and pre-packaged viral libraries at 2 x 10^8 TU and 1 x 10^9 TU

 

  • Designed to work with activated spCas9 (dCas9) complexed with an activator (dCas9-VPH) to activate gene expression
  • Library includes 3700 non-targeting sgRNA controls
  • Targets nearly 19,000 human coding regions
  • Pooled library of ~105,000 dual-sgRNA constructs each targeting a single gene
  • 5 CRISPRa dual-sgRNA constructs target each gene
  • Increases the levels of gene activation by 10-fold in comparison to the single-sgRNA library

  • Targeted Gene Activation

  • Gain of Function studies

  • Functional Genetic Screening

  • Identification of Novel Targets for Therapeutics

  • Biomarker Analysis

    

Cat # 

Product Name 

Size 

KIDHGW-105K-P

Human Genome-Wide CRISPRiDual-sgRNA Library (plasmid)

200 ug ​

KIDHGW-106-V8

Human Genome-Wide CRISPRiDual-sgRNA Library (virus)

2 x 10^8 TU ​

KIDHGW-106-V9

Human Genome-Wide CRISPRiDual-sgRNA Library (virus)

1 x 10^9 TU ​

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Human Genome-Wide CRISPRi Dual-sgRNA Libary