DriverMap™ AIR TCR/BCR Profiling Kits

Cellecta's DriverMap™ Adaptive Immune Receptor (AIR) Profiling Kits provide a sensitive and comprehensive approach to characterizing T-cell receptor (TCR) and B-cell receptor (BCR) repertoires from bulk samples. Combining highly multiplexed PCR or RT-PCR with next-generation sequencing (NGS), these assays enable researchers to identify and quantify immune receptor clonotypes, assess repertoire diversity, and investigate clonal expansion and changes in adaptive immune responses.

DriverMap AIR assays are available for human and mouse RNA or DNA samples. RNA-based assays profile expressed TCR/BCR repertoires, enabling sensitive detection of low-abundance clonotypes, while DNA-based assays assess receptor gene rearrangements to estimate clonal frequencies. Depending on the assay, researchers can analyze CDR3 sequences or longer receptor regions. Cellecta's proprietary Validator Barcodes (VBCs) provide molecular-level information that supports accurate and reproducible clonotype quantification while helping account for PCR amplification bias.

Key Features and Benefits

Comprehensive TCR/BCR Profiling: Assay options for the four TCR chains (TRA, TRB, TRG, TRD) and three BCR chains (IGH, IGK, IGL).
RNA and DNA Analysis: Profile expressed receptor repertoires or genomic clonotype frequencies, with complementary information available from parallel RNA and DNA analysis.
Sensitive Clonotype Detection: Identify low-abundance receptor sequences using targeted multiplex amplification and NGS.
Reliable Quantification: Validator Barcodes help distinguish original template molecules from PCR amplification products, improving clonotype abundance estimates.
Flexible Sequencing Options: Assay-dependent options for CDR3 or longer receptor-region analysis.
Streamlined NGS Workflow: Prepare sequencing libraries using standard molecular biology equipment and compatible UDI NGS index plates.